Saba banana (Musa balbasiana) is a nutritious and the most popular banana in the Philippine market. Once utilized, its fruit generates a huge amount of agricultural waste. On the other hand, the paddy straw mushroom (Volvariella volvacea) was known as one of the most cultivated mushrooms in the world due to its nutrients and pleasant flavor. This study aimed to determine the paddy straw mushroom mycelial production in banana (peeling, leaves, and pseudostem) wastes media. Banana wastes media were prepared by decocting banana parts tap water to produce 50% and 100% concentrations of decoction, adding 20 g of white gulaman bar and 10 g of white table sugar until the homogenous solution was attained; pH was adjusted into pH 8 using NaOH and HCl. For sterilization, the media was autoclaved for 20 minutes at 121°C /15 psi. Mycelial growth and density of V.volvacea were evaluated in different banana wastes media namely: T1: Coconut water, T2: Decocted Banana Peeling (50%), T3: Decocted Banana Peeling (100%), T4: Decocted Banana Leaves (50%), T5: Decocted Banana Leaves (100%), T6: Decocted Banana Pseudo stem (50%) and T7: Decocted Banana Pseudo stem (100%). Data was analysed using the Completely Randomized Design. At an average temperature of 28.6°C and 74.77% relative humidity, paddy straw mushrooms showed the highest mycelial run in T4 compared to other banana wastes media. The T2 and T3 were noted with the thickest mycelia among all evaluated media. T4 was recorded with the shortest period of ramification within 3 days and was considered as the ideal culture media for paddy straw mushroom among banana wastes media since the faster the mycelial grows, the quicker the mushroom production. Among the media tested, the T4- 50% of banana leaves extract is the ideal alternative media for V. volvacea mycelial production.
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